Invitrogen™ Human MET [M1250T], His Tag Recombinant Protein

Catalog No.	Quantity
PV4272	1 mg
PR7432A	100 μg

Catalog No. PV4272 Supplier Invitrogen™ Supplier No. PV4272

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Cytoplasmic

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

Specifications

• Accession Number: NP_000236.2, M1250T
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 2mM DTT, 50% glycerol, 0.02% Triton X-100, 150mM NaCl, 0.5mM EDTA and no preservative; pH 7.5
• Molecular Weight (g/mol): 53.7 kDa
• Name: Human MET [M1250T], His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: MET M1250T
• Common Name: MET (M1250T Mutant)
• Kinase Family: MET (HGFR) Kinase Family
• Protein Length: 956-1390; M1250T
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: 956-1390; M1250T
• Content And Storage: Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Cytoplasmic
• Form: Liquid
• Research Category: Drug Development