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Zymo Research Corporation Methylated & Non-methylated pUC19 DNA Set, 20 ng
Supplier: Zymo Research Corporation D5017
The Non-Methylated pUC19 DNA is pUC19 that was isolated from a methylation-negative strain of bacteria (Dam–, Dcm–) and can be used as a negative control for DNA methylation analysis. The Methylated pUC19 DNA is pUC19 that has been isolated from the same strain and has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2(EC 2.1.1.37; Figure 1) and can be used as a positive control for DNA methylation analysis. The primer set herein has been designed to amplify a fragment of the supplied pUC19 DNAs following bisulfite treatment. The methylated cytosines, comprising CG dinucleotides in the Methylated pUC19 DNA remain unconverted following bisulfite treatment, whereas nonmethylated cytosines are converted into uracil and detected as thymine after PCR. The supplied pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.
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