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Apexbio Technology LLC Micro BCA Protein Assay Kit 2000T
Supplier: Apexbio Technology LLC K4102500
Bicinchoninic Acid Assay (BCA) is a protein quantification method based on the biuret reaction Under alkaline conditions protein peptide bonds reduce Cu2 ions from copper sulfate to Cu subsequently forming a purple-colored complex by chelating Cu with BCA which strongly absorbs at a wavelength of 562 nm The color intensity of the chelate is correlated with protein concentration while the reduction of Cu2 to Cu is directly proportional to the amount of protein present in the solution Thus protein concentration can be determined by measuring the absorbance This kit is specifically designed to quantify low-concentration protein samples measuring total protein concentrations in the range of 0 5 20 g/mL The kit features ease of operation excellent stability high sensitivity and wide compatibility It exhibits good compatibility with many ionic and non-ionic detergents
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