Applied Biosystems™ MicroSEQ™ 500 16S rDNA Sequencing Kit

Description

The sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria.

• The sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria
• Use this kit to sequence PCR products that have been generated using the MicroSEQ™ 500 16S rDNA PCR Kit
• The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene
• Sequences can then be compared to the MicroSEQ™ 16S rDNA 500 Library to generate an identity match
• This kit does not include a protocol or a Quick Reference Card
• For Research Use Only. Not for use in diagnostics procedures.
• Store at -15° to -25°C

Benefits of the MicroSEQ™ 500 16S rDNA Sequencing Kit

• Sequence-based measurement ensures you get reproducible results in under 24 hours
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria
• Universal domain-level primers eliminate up-front optimization
• MicroSEQ™ ID Analysis Software ensures accurate species identification
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms
• Requires minimal training-same work flow as the MicroSEQ™ D2 LSU fungal kit

Specifications

• Content And Storage: Each kit contains:
  Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.
• Detection Method: Capillary Electrophoresis
• Format: Kit
• Sample Type: PCR Products
• For Use With (Equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
• For Use With (Application): Sequencing
• Includes: Forward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)
• Libraries: Customizable, Bacterial Library (> 2000 species)
• No. of Reactions: 55 Forward, 55 Reverse
• Product Line: MicroSEQ™
• Product Type: Sequencing Kit
• Quantity: 1 Kit
• Software: MicroSEQ™ ID Analysis Software
• Shipping Condition: Dry Ice
• Target: 1st 500 bp of 16S rRNA gene
• Target Organism Class: Bacteria
• Technique: SBT (Sequence-Based Typing)
• Workflow Step: Cycle Sequencing

For Research Use Only. Not for use in diagnostic procedures.