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Applied Biosystems™ MicroSEQ™ ITS rDNA Fungal Identification Kit

Catalog No. A40005277
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PrepMan™ Ultra Sample Preparation Reagent
PrepMan™ Ultra Sample Preparation Reagent and Cleanup Reagents
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A40005277 Kit Only
A40005278 PrepMan™ Ultra Sample Preparation Reagent and Cleanup Reagents
A40005279 PrepMan™ Ultra Sample Preparation Reagent
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Catalog No. A40005277 Supplier Applied Biosystems™ Supplier No. A40005277
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The MicroSEQ™ ITS rDNA Fungal Identification Kit is a comprehensive solution including both PCR and sequencing components. It provides an easy-to-use, DNA sequence-based method to identify most fungi with high accuracy and objective results.

The MicroSEQ™ ITS rDNA Fungal Identification Kit is a comprehensive solution including both PCR and sequencing components. It provides an easy-to-use, DNA sequence-based method to identify most fungi with high accuracy and objective results.

Key Features:

  • High-Resolution differentiation: Targets the highly variable ITS1 and ITS2 spacers to differentiate between 'sibling species' fungi that are genetically similar but carry vastly different risk profiles in cleanroom environments.
  • Primary barcode: Utilizes the primary barcode for fungal identification, enabling alignment with global phylogenetic standards.
  • Objective Identification: Growth-independent measurement enables fungal identification based on objective genetic criteria rather than subjective phenotypic traits.
  • High-Confidence Results: Powered by MicroSEQ™ ID Analysis Software to enable accurate, species-level identification.
  • Flexible Database Management: Customizable libraries allow you to create proprietary databases or search validated libraries containing the most frequently encountered organisms.
  • Simplified Training: Utilizes the same intuitive workflow as the MicroSEQ™ Full Gene 16S rDNA Bacterial Identification Kit, minimizing the learning curve for lab personnel.

Specific and Easy to Use

By leveraging phylogenetic analysis of the full Internal Transcribed Spacer (ITS) region comprising the ITS1 and ITS2 non-coding region, this kit enables the identification of even the most challenging fungi and yeasts. To enable precision, the software compares your samples against a curated database of ITS sequences spanning over 22,000 species.

The MicroSEQ™ ID System: A Complete, Integrated Solution

The Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, thermal cyclers and sequencing systems. To enable optimal performance and system-wide integration, we develop and test all components of the system together.

Specifications

Detection Method Sequencing
Format Kit
Isolation Technology Tube Centrifugation
Sequencing Type DNA and RNA Sequencing
For Use With (Equipment) 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer,SEQStudio, SEQStudio Flex, GeneAmp 9700, Veriti Thermal Cycler
For Use With (Application) Sequencing
Includes Kit Only
Libraries MicroSEQ ID Fungal ITS Gene Library
No. of Reactions 60 Reactions
Product Line MicroSEQ
Product Type PCR and Sequencing Kit
Quantity 1 Ea.
Software MicroSEQ™ ID Analysis Software
Shipping Condition Dry Ice
Target Organism Class Fungi
Technique SBT (Sequence-Based Typing)
Workflow Step Cycle Sequencing
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For Research Use Only.

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