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Hardy Diagnostics™ Middlebrook 7H9 Broth for Mycobacteria
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Catalog No. 23001735 Shop All Hardy Diagnostics Products
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Species:
Mycobacterium
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Catalog No. Species
23-001-735 Mycobacterium
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Catalog No. 23-001-735 Supplier Hardy Diagnostics™ Supplier No. C32
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Description

For the cultivation of Mycobacterium spp. from sterile body sites.

  • Not intended to be used for the diagnosis of human disease
  • The basal medium of 7H9 broth is supplemented with Middlebrook ADC Enrichment, which is composed of bovine albumin, dextrose, and catalase
    • Albumin acts as a protective agent and binds free fatty acids that may be toxic to Mycobacterium spp
    • Dextrose serves as an energy source
    • Toxic peroxides that may be present in the medium are destroyed by catalase
  • Glycerol in the broth provides an abundant source of carbon and energy for the tubercle organisms

Specifications

Appearance Clear and Colorless
Broth Type 7H9 Broth
Cap Color Black
Certifications/Compliance CLSI Document M22-A3
Color Clear and Colorless
Content And Storage 2°C to 8°C
Additive Glycerol, Biotin, Sodium Citrate, Bovine Albumin, Dextrose, Beef Catalase
Detects Mycobacterium spp.
Format Liquid
Media Type Middlebrook 7H9 Broth
Product Type Broth Tube
Sterility Sterile
Target Organism Class Mycobacteria
Test Type Microbial Cultivation
Fill Volume 10 mL
Formulation L-Glutamic Acid - 0.5g, Ammonium Sulfate - 0.5g, Sodium Citrate - 0.1g, Magnesium Sulfate - 50.0mg, Ferric Ammonium Citrate - 40.0mg, Zinc Sulfate - 1.0mg, Copper Sulfate - 1.0mg, Pyridoxine - 1.0mg, Calcium Chloride - 0.5mg, Biotin - 0.5mg, Glycerol - 2.0mL.ADC Enrichment - Bovine Albumin - 5.0g, Dextrose - 2.0g, Beef Catalase - 3.0mg
For Use With (Application) Cultivation of Mycobacterium spp., Preparing dilutions of mycobacteria for antimicrobial testing
Incubation Conditions 35 ±2°C in 5-10% CO2 atmosphere, Protect from Light
Incubation Time 8 Weeks
No. per Pack 20
Packaging Type Box
Quantity 20 x 10 mL
Volume (Metric) Specimen 10 mL
Dimensions (Dia. x H) 16 x 125 mm
Height (Metric) 125 mm
Diameter (Metric) 16 mm
Species Mycobacterium
Protein Source Bovine, Beef
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