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Sigma Aldrich Fine Chemicals Biosciences Minimum Essential medum E500ML
Supplier: Sigma Aldrich Fine Chemicals Biosciences M465524X500ML
Minimum Essential Medium (MEM) developed by Harry Eagle is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagles Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.MEM which incorporates these modifications includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks or Earles salts has broadened the usefulness of this medium.
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