Invitrogen™ Mouse MIP-2/CXCL2 ELISA Kit

Catalog No.	Quantity
EMCXCL2X5	5 x 96 Tests
EMCXCL2	96 Tests

Catalog No. EMCXCL2X5 Supplier Invitrogen™ Supplier No. EMCXCL2X5

Description

ELISA

The Mouse MIP-2 (CXCL2) ELISA quantitates Ms MIP-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-2. Principle of the method The Mouse MIP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

Specifications

• Accession Number: P10889
• Assay Range: 10-2000 pg/mL
• Assay Sensitivity: 10 pg/mL
• Calibration Range: 10 pg/mL to 2000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Sufficient For: 5 x 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 2; CINC-2a; Cinc3; CINC-3; CXC; CXC motif chemokine; C-X-C motif chemokine 2; C-X-C motif chemokine ligand 2; CXCL; CXCL2; Cytokine-induced neutrophil chemoattractant 3; GRO b; gro beta; GRO β; GRO2; GRO2 oncogene; GROb; GRObeta; gro-beta; GRO-beta(5-73); GRO-beta-T; Growth-regulated protein beta; GROβ; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; Mgsa-b; MIP 2 a; MIP 2 alpha; MIP 2 α; Mip2; MIP-2; MIP2 a; MIP2 α; MIP2A; MIP-2a; MIP2-alpha; SB-251353; Scyb; Scyb2; small inducible cytokine subfamily, member 2C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 2; CINC-2a; Cinc3; CINC-3; CXC; CXC motif chemokine; C-X-C motif chemokine 2; C-X-C motif chemokine ligand 2; CXCL; CXCL2; Cytokine-induced neutrophil chemoattractant 3; GRO b; gro beta; GRO β; GRO2; GRO2 oncogene; GROb; GRObeta; gro-beta; GRO-beta(5-73); GRO-beta-T; Growth-regulated protein beta; GROβ; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; Mgsa-b; MIP 2 a; MIP 2 alpha; MIP 2 α; Mip2; MIP-2; MIP2 a; MIP2 α; MIP2A; MIP-2a; MIP2-alpha; SB-251353; Scyb; Scyb2; small inducible cytokine subfamily, member 2; CINC-2a; GROb; Gro2; MIP-2; MIP-2a; Mgsa-b; Mip2; Scyb; Scyb2
• Gene ID (Entrez): 20310
• Gene Symbol: Cxcl2
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Macrophage inflammatory protein 2, MIP2
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: CINC-2 A, GROb, Gro2, MIP-2, MIP-2 A, Mgsa-b, Mip2, Scyb, Scyb2
• Target: CXCL2
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min