Invitrogen™ Human MIP-3 alpha/CCL20 Cell Lysate ELISA Kit

Description

ELISA

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α. Principle of the method The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

Specifications

• Accession Number: P78556
• Assay Range: 0.8 to 600 pg/mL
• Assay Sensitivity: 1.5 pg/mL
• Calibration Range: 1.5 pg/mL to 600 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Chemokine & Receptors
• Sample Type: Cell Lysates
• Specificity: The antibody pair provided in this kit recognizes human MIP-3 alpha.
• Sufficient For: 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6364
• Gene Symbol: CKb4,Exodus,LARC,MIP-3-alpha,MIP-3 A,MIP3A,SCYA20,ST38
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: CKb4, Exodus, LARC, MIP-3-alpha, MIP-3 A, MIP3A, SCYA20, ST38
• Target: CCL20
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins