Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. 502895984

Cayman Chemical MitoCheck Complex II/III Activity Assay Kit, 96 Well
SDP

Catalog No. 502895984
Encompass_Preferred

Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation.; It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone.; Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle.; During the Q-cycle, electrons are passed from QH2 onto cytochrome c via the Rieske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-.; Cayman's MitoCheck Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III.; This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2.; Due to the dependence on complex II activity, a counter-screen for complex II activity (Item No.; 700940) should be performed in order to truly measure complex III activity.

Catalog No. 50-289-5984 Supplier Cayman Chemical Supplier No. 70095096 WELL
May include imposed supplier surcharges.
Add to Cart
Add to Cart

missing translation for 'validMainframeMsg'

Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation.; It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone.; Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle.; During the Q-cycle, electrons are passed from QH2 onto cytochrome c via the Rieske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-.; Cayman's MitoCheck Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III.; This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2.; Due to the dependence on complex II activity, a counter-screen for complex II activity (Item No.; 700940) should be performed in order to truly measure complex III activity.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.