This labeling kit is for labeling 50-100 mug antibody with the green fluorescent dye Cf488A. Mix-n-Stain Cf dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior Cf dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Cf488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection settings Cf488A is similar to Alexa fluor(R) 488 in brightness and photostability. In microscopy or flow cytometry applications using a 520/20 filter Cf488A may appear brighter than Alexa fluor(R) 488 due to its shorter emission wavelength which makes the dye optically more compatible. The shorter wavelength of Cf488A also minimizes the dyes spill-over into longer wavelength channels making the dye advantageous in multi-color detection. Like Alexa fluor(R) 488 Cf488A is highly water soluble but is not