5 doses of 10 µg/kg total body weight of Neupogen (filgrastim) are administered over 5 consecutive days prior to collection. Leukapheresis is performed on the day of the 5th administration and the following day* using the Spectra Optia Apheresis System. Mononuclear cell-rich blood is continuously collected using ACD-A as an anticoagulant. Approximately 120mL of autologous plasma is com-bined with apheresed cells. The product is filled to deliver a nominal 290mL and is shipped in a 300mL transfer bag. Total nucleated cells (TNC) in our fresh Neupogen-mobilized leukapheresis products are composed of, on average, 0.9% CD34+ hematopoietic stem and progenitor cells (HSPCs). Actual TNC concentration and CD34+ percentage vary based on donors’ response to mobilization and from collection to collection. *Second bag from the donor is for sale only when First bag is purchased.