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Reinforced Clostridial Broth (RCB) is an enriched non-selective medium intended for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.RCB is a enriched non-selective broth medium formulated by Hirsch and Grinstead to enhance the growth of clostridia from a small inoculum. Casein, beef extract, and yeast extract form the nutritive base that supplies a source of carbon, nitrogen, vitamins, and minerals. Dextrose is added as a source of carbohydrates. L-Cysteine is included as a reducing agent. Soluble starch is a detoxifier of metabolic by-products while sodium acetate acts as a buffer. Resazurin is added as an indicator of significant oxygen exposure to the media.