Invitrogen™ Click-IT™ GlcNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetylglucosamine)

Description

Compatible with LC-MS/MS and Multiplexed Proteomics™ technologies

The Click-iT GlcNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins. In step one, cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then detected with a Click-iT Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). The Click-iT glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.

• Less hazardous

Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Mass Spectrometry, Metabolic Labeling, Peptide Labeling, Protein and Antibody Labeling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Green Features: Less hazardous
• Product Type: Labeling Reagent
• Quantity: 5.2 mg
• Content And Storage: Store in freezer -5°C to -30°C.
• Detection Method: Biotin-based, Fluorescence
• Shipping Condition: Room Temperature
• Product Line: Click-iT™
• Label or Dye: Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
• Labeling Target: Proteins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.