Invitrogen™ ImaGene Red™ C₁₂RG lacZ Gene Expression Kit

Description

Substrates cleaved within cell for fluorescent product

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C₁₂RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C₁₂RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.

Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays

Order Info

Shipping Condition: Room Temperature

Specifications

• Product Type: β-Galactosidase Reporter Gene Assay System
• Detection Method: Fluorescence
• Assay: β-Gal (lacZ) Assay
• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Shipping Condition: Room Temperature
• Substrate: C₁₂RG
• Substrate Type: Beta-Gal Substrate
• Substrate Properties: Chemical Substrate
• Target Enzyme: Beta-Galactosidase
• Format: Kit
• Product Line: DetectaGene
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.