Invitrogen™ PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)

Description

PED-A1-labeled phospholipid

Molecular Probes phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells.

• Label (Ex/Em): PED-A1
• Specificity: PLA₁
• Cleavage product: BODIPY™ FL C5
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry
• Cell-Permeant

Note: Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

• Cell Permeability: Cell-Permeant
• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• Form: Solid
• Product Line: BODIPY™
• Quantity: 100 μg
• Substrate: Phospholipase A1 selective substrate
• Substrate Properties: Lipid-Based Substrate
• Target Enzyme: Phospholipase
• For Use With (Equipment): Flow Cytometer, Microplate Reader
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.