Invitrogen Molecular Probes Red/Green BODIPY PC-A2 (1-O-(6-BODIPY 558/568-Aminohexyl)-2-BODIPY FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)
Measure results in microplate assays or using flow cytometry
Manufacturer: Invitrogen A10072
Molecular Probes phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.
- Label (Ex/Em): Red/Green BODIPY PC-A2 (approx. 488/530,590nm) (can be used in dual emission fluorescence ratio detection)
- Specificity: PLA2
- Cleavage product: BODIPY FL C5
- Lyophilized product can be dissolved in DMSO for use
- Fluorescence is measured either in microplate assays or using flow cytometry
Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis
Shipping Condition: Room temperature
|Phospholipase A Substrate|
|Store in freezer (-5° to -30°C).|
|Flow Cytometer, Microplate Reader|
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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.