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Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)
Description
Molecular Probes phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.
- Label (Ex/Em): Red/Green BODIPY™ PC-A2 (approx. 488/530,590nm) (can be used in dual emission fluorescence ratio detection)
- Specificity: PLA2
- Cleavage product: BODIPY FL C5
- Lyophilized product can be dissolved in DMSO for use
- Fluorescence is measured either in microplate assays or using flow cytometry
Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Cell Permeability | Cell-Permeant |
| Content And Storage | Store in freezer (-5 to -30°C). |
| Detection Method | Fluorescence |
| Form | Solid |
| Product Line | BODIPY |
| Quantity | 100 μg |
| Substrate | Phospholipase A2 substrate (ratiometric) |
| Substrate Properties | Lipid-Based Substrate |
| Target Enzyme | Phospholipase |
| For Use With (Equipment) | Flow Cytometer, Microplate Reader |
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For Research Use Only. Not for use in diagnostic procedures.
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