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Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)

Catalog No. R22124
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R22124 2 mg
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Catalog No. R22124 Supplier Invitrogen™ Supplier No. R22124
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Description

This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.

This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells. These fluorogenic substrates contain an amino acid or peptide covalently linked to each of R110's amino groups. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted first to the fluorescent monoamide and then to R110, with a further increase in fluorescence. The substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader, or can be used for intracellular protease assays with analysis by flow cytometry or fluorescence microscopy.<

• Has spectral properties similar to those of fluorescein, making it compatible with standard fluorescein filter sets
• Signal to background ratios are good because of large extinction coefficient of R110
• Unlike fluorescein, the fluorescence intensity of the rhodamine 110 reaction product is constant from pH 3–9
• Substrate name: p-tosyl-Gly-Pro-Arg)2-R110
• Useful to measure: Thrombin
• Ex/Em of R110 product: (498/521nm)
• Cell-Permeant

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Permeability Cell-Permeant
Content And Storage Store in freezer (-5 to -30°C).
Detection Method Fluorescence
Form Solid
Quantity 2 mg
Substrate Caspase Substrate
Substrate Properties Peptide-Based Substrate
Target Enzyme Thrombin
For Use With (Equipment) Fluorometer, Microplate Reader
Shipping Condition Room Temperature

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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