Edvotek™ Molecular Weight Determination of Proteins

Description

Includes

instructions, prestained LyphoProtein™ samples, gel loading solution, agarose, electrophoresis buffer, stain, SDS solution

Requires

• Required Materials (not included):
• Horizontal gel electrophoresis apparatus
• DC power supply
• Waterbath
• Visualization system (white light)
• Automatic micropipets with tips
• Pipet pump
• 500mL flasks
• Distilled or deionized water
• Methanol
• Glacial acetic acid
• Microwave
• Hotplate or Bunsen burner

Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis.

Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is EDVOTEK™ ́s formulation of protein grade agarose, which provides an alternative to the use of polyacrylamide gels.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Determine protein subunit molecular weights using horizontal electrophoresis
• Includes: Instructions, pre-stained LyphoProtein™ gel marker, pre-stained LyphoProtein™ samples, practice gel loading solution, Protein Agarose™ powder, Tris-Glycine-SDS powdered buffer, Protein InstaStain™, transfer pipets, and 1 mL pipet
• Requires: Horizontal electrophoresis apparatus, power supply, micropipette with tips, microwave oven or hot plate, 250 mL or 1 L flask, larger beaker, lab tape or markers, hot gloves, safety goggles and lab gloves, and distilled or deionized water. For Staining with Protein InstaStain™ (optional): methanol, glacial acetic acid, small plastic tray or large weigh boat, plastic wrap, white light box (recommended), rocking platform, and pipet pump or bulb
• Time Range: Gel Prep: 40 minutes; Electrophoresis: 60 minutes
• Content And Storage: Some components require freezer storage.
• Class Size: 6 Lab Groups