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New England Biolabs, Inc. Monarch RNA Cleanup Kit (500 µg) – 10 preps
Supplier: New England Biolabs, Inc. T2050S
The Monarch RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience.
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