New England Biolabs, Inc. Monarch RNA Cleanup Kit (50 µg) – 10 preps

Supplier:  New England Biolabs, Inc. T2040S

The Monarch RNA Cleanup Kit (50 ug) rapidly and reliably purifies up to 50 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in downstream applications including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch RNA Cleanup kits are also available for 10 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience.

Catalog No. NC1549467