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Invitrogen™ Mouse anti-Bovine Ig (Light chain) Secondary Antibody

Catalog No. 01676135
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100 μg
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01-676-135 100 μg
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Catalog No. 01-676-135 Supplier Invitrogen™ Supplier No. MA119384
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Description

Mouse Monoclonal Secondary Antibody

This antibody reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. Western Blot: Overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.

Anti-Bovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for bovine (cow, ox) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Bovine Ig (Light chain)
Applications Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
Classification Monoclonal
Clone IVA285-1
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 15mM sodium azide; pH 7.4
Gene Alias Iglc
Host Species Mouse
Immunogen Fraction of bovine immunoglobulin.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Bovine, Sheep
Content And Storage 4°C, do not freeze
Product Type Secondary Antibody
Form Liquid
Isotype IgG1
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