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Description
- Mouse anti HA-Tag mAb offers high specificity and potency and consistency against HA tag, which has been experimentally validated in Western blotting (WB), Immunoprecipitation (IP), and Immunofluorescence (IF) assays.
- Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing.
- Tags are attached to proteins for various purposes. Epitope tags are short peptide sequences that are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag, and NE-tag. These tags are particularly useful for western blotting, immunofluorescence, and immunoprecipitation experiments, although they also find use in antibody purification.
Specifications
Specifications
| Antigen | HA-Tag |
| Applications | Western Blot, Immunoprecipitation, Immunofluorescence |
| Classification | Monoclonal |
| Concentration | WB: 1:2000 - 1:5000; IF: 1:50 - 1:500; IP: 1:20 - 1:50 |
| Conjugate | Unconjugated |
| Formulation | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol; pH 7.3 to 7.4 |
| Host Species | Mouse |
| Immunogen | Synthetic peptide corresponding to HA tag |
| Purification Method | Affinity Purification |
| Quantity | 100 μL |
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Safety and Handling
Unless specified otherwise, for research or further manufacturing use only, not for direct human use.
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