Invitrogen™ Mouse anti-Human Kappa Light Chain Secondary Antibody, Biotin

Mouse Monoclonal Secondary Antibody

Supplier:  Invitrogen™ MA512114

Catalog No. PIMA512114

Description

By Western blot, MA5-12114 detects Human Ig kappa light chain chain at ~25kD in human samples. At high concentrations, the antibody will also detect heavy chain. By ELISA and WB, MA5-12114 does not react with human Ig lambda light chain.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human Kappa Light Chain
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunoprecipitation, Flow Cytometry, ELISA
• Clone: L1C1
• Conjugate: Biotin
• Gene Alias: Immunoglobulin kappa light chain, Ig kappa light chain, Ig kappa, kappa light chain, Igk
• Immunogen: B lymphoma cells.
• Quantity: 500 μL
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid