Recombinant Protein;1MG;Mouse B7-1, Fc Tag (CD0-M5259) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.3 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 2 μg/mL (100 μL/well) can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.05-0.8 ng/mL (QC tested).;Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 5 μg/mL (100 μL/well) can bind Mouse PD-L1, mouse IgG2a Fc tag, low endotoxin (Cat. No. PD1-M52A2) with a linear range of 0.16-2.5 μg/mL (Routinely tested).;Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) captured on CM5 chip via anti-human IgG Fc antibody can bind Mouse CD28, His Tag (Cat. No. CD8-M52H6) with an affinity constant of 49.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).