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  5. Invitrogen™ Mouse BCL-W ELISA Kit 

Invitrogen™ Mouse BCL-W ELISA Kit

Catalog No. EM8RBX5
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Description

ELISA

Mouse BCL-W quantitates mouse BCL-W in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse BCL-W.

Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. Therox portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Apoptosis regulator Bcl-W; AW048834; BCL2 like 2; BCL2L2; bcl2-L-2; Bcl2-like 2; BCL2-like 2 protein; Bcl-2-like protein 2; Bcl2-like protein 2; Bclw; BCL-W; BCL-WEL; BCL-WS; c98; Gtrgal2; Gtrosa41; KIAA0271; PPP1R51; protein phosphatase 1, regulatory subunit 51
Assay Range 0.41 to 100 ng/mL
Assay Sensitivity 0.41 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 12050
Gene Symbol BCL2L2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target BCL-W
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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