Invitrogen™ Mouse Collagen I ELISA Kit

Catalog No.	Quantity
EEL218	96 Tests

Catalog No. EEL218 Supplier Invitrogen™ Supplier No. EEL218

Description

ELISA

Mouse Collagen I ELISA Kit quantifies Collagen I in serum and plasma. The Mouse Collagen I solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

Specifications

• Accession Number: P11087, Q01149
• Assay Range: 0.31-20 ng/mL
• Assay Sensitivity: 0.19 ng/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AA960264; AI325291; alpha 1 type I collagen; alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) collagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha1 chain of type I collagen; alpha-1 collagen (I); alpha-1 type 1 collagen; alpha-1 type I collagen; alpha-1 type-1 collagen; alpha1(I) procollagen; alpha2 chain of type I collagen; alpha-2 type I collagen; alpha-2(1) collagen; alpha2(I) collagen; COL1; Col1a1; Col1a-1; COL1A1; matrix protein; Col1a2; Col1a-2; COLA; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen; collagen 1 alpha 2 chain; collagen a2; collagen alpha 1 chain type I; collagen alpha 2(I) chain precursor; collagen alpha-1 chain; collagen alpha-1 chain precursor; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen alpha-2; collagen alpha-2 chain precursor; collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen type I alpha 1; collagen type I alpha 1 chain; collagen type I alpha 2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; EDSC; Mov13; Mov-13; OI1; OI2; OI3; OI4; oim; osteogenesis imperfecta; precollagen alpha-2; prepro-alpha-1 collagen type I; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; similar to pro alpha 1(I) collagen; Type I Collagen; type I collagen alpha 1 chain; type I collagen alpha1; type I collagen alpha-2 chain; type I collagen pre-pro-alpha1(I) chain; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagenAA960264; AI325291; alpha 1 type I collagen; alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) collagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha1 chain of type I collagen; alpha-1 collagen (I); alpha-1 type 1 collagen; alpha-1 type I collagen; alpha-1 type-1 collagen; alpha1(I) procollagen; alpha2 chain of type I collagen; alpha-2 type I collagen; alpha-2(1) collagen; alpha2(I) collagen; COL1; Col1a1; Col1a-1; COL1A1; matrix protein; Col1a2; Col1a-2; COLA; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen; collagen 1 alpha 2 chain; collagen a2; collagen alpha 1 chain type I; collagen alpha 2(I) chain precursor; collagen alpha-1 chain; collagen alpha-1 chain precursor; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen alpha-2; collagen alpha-2 chain precursor; collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen type I alpha 1; collagen type I alpha 1 chain; collagen type I alpha 2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; EDSC; Mov13; Mov-13; OI1; OI2; OI3; OI4; oim; osteogenesis imperfecta; precollagen alpha-2; prepro-alpha-1 collagen type I; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; similar to pro alpha 1(I) collagen; Type I Collagen; type I collagen alpha 1 chain; type I collagen alpha1; type I collagen alpha-2 chain; type I collagen pre-pro-alpha1(I) chain; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen;
• Gene ID (Entrez): 12842, 12843
• Gene Symbol: COL1A1, COL1A2
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Collagen I
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min