Invitrogen™ Mouse EPO ELISA Kit

Catalog No.
EM28RBX10	10 x 96 Tests; HRP

Catalog No. EM28RBX10 Supplier Invitrogen™ Supplier No. EM28RBX10

Description

ELISA

Mouse EPO quantitates mouse EPO in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse EPO.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Accession Number: P07321
• Assay Range: 1.5-400 pg/mL
• Assay Sensitivity: 1.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2;
• Gene ID (Entrez): 13856
• Gene Symbol: EPO
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Epo
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min