Invitrogen™ Mouse Estradiol Rapid ELISA Kit

Catalog No. EELR013

Supplier: Invitrogen™ EELR013

Description

ELISA

The Mouse Estradiol (E2) ELISA quantitates E2 in Serum, Plasma, Urine, Saliva. Principle of the method The Mouse E2 solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target based on competitive binding. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Mouse E2 in the samples competes with the pre coated Mouse E2 for binding sites on the Horseradish Peroxidase (HRP) linked detection antibody. Excess conjugate and unbound sample or standard are washed away. A substrate solution is added that reacts with the enzyme antibody target complex to produce a measurable signal. The intensity of this signal, measured as optical density (OD) at a wavelength of 450±2 nm, is inversely proportional to the concentration of target present in the original specimen. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale wit...

Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.

Specifications

• Assay Range: 3.13-200 pg/mL
• Assay Sensitivity: 1.88 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Saliva, Serum, Urine
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 17-Beta-Estradiol; 17-beta-HSD17-Beta-Estradiol; 17-beta-HSD;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Saliva 50 μL, Serum 50 μL, Urine 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Estradiol
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min