Invitrogen™ Mouse GM-CSF Recombinant Protein

Catalog No.	Quantity
PIRMGMCSF20	20 μg

Catalog No. PIRMGMCSF20 Supplier Invitrogen™ Supplier No. RMGMCSF20

Description

Recombinant Protein

This product cross-reacts with rat, mouse, trout, bacteria, leech, and human GM-CSF. Use: Mouse GM-CSF exerts activity in the range of 0.05 to 0.5 ng/mL for most in vitro applications. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 μg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

Specifications

• Accession Number: P01587
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 12981
• Molecular Weight (g/mol): 14.2 kDa
• Name: Mouse GM-CSF
• Quantity: 20 μg
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostim
• Common Name: GM-CSF
• Gene Symbol: CSF2
• Biological Activity: ED50 ≤ 0.05 ng/mL; determined by the dose-dependent proliferation of mouse FDC-P1 cells.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: >98% by SDS-PAGE and RP-HPLC

Safety and Handling

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