Invitrogen™ Mouse HAMP ELISA Kit

Catalog No.	Quantity
EEL101	96 Tests

Catalog No. EEL101 Supplier Invitrogen™ Supplier No. EEL101

Description

ELISA

The Mouse Hepcidin ELISA quantitates Hepcidin in serum, plasma, and other biological fluids The Mouse Hepcidin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

HAMP is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity.

Specifications

• Accession Number: Q9EQ21
• Assay Range: 125-8000 pg/mL
• Assay Sensitivity: 75 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Hepcidin
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: HAMP; Hamp1; HEPC; Hepc1; Hepc20; Hepc25; Hepcidin; hepcidin antimicrobial peptide; hepcidin antimicrobial peptide 1; Hepcidin-20; Hepcidin-25; HFE2B; LEAP1; LEAP-1; liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor; UNQ487/PRO1003HAMP; Hamp1; HEPC; Hepc1; Hepc20; Hepc25; Hepcidin; hepcidin antimicrobial peptide; hepcidin antimicrobial peptide 1; Hepcidin-20; Hepcidin-25; HFE2B; LEAP1; LEAP-1; liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor; UNQ487/PRO1003;
• Gene ID (Entrez): 84506
• Gene Symbol: HAMP
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: HAMP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min