Gibco™ Mouse IFN-alpha A Recombinant Protein

Catalog No.	Quantity
PMC4016	1 x 10^5 units

Catalog No. PMC4016 Supplier Gibco™ Supplier No. PMC4016

Description

Recombinant Protein

Dilution Recommendation: Polypropylene tubes and pipet tips should be used when diluting this interferon. Further dilutions should be made in buffered solution containing a carrier protein, such as BSA. The optimal concentration should be determined for each specific application.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01573
• For Use With (Application): Bioactivity
• Formulation: PBS with 0.1% BSA and no preservative
• Gene ID (Entrez): 15965
• Molecular Weight (g/mol): 19.3 kDa
• Name: Mouse IFN-alpha A
• Purification Method: Purified
• Quantity: 1 x 10^5 units
• Storage Requirements: -80°C
• Regulatory Status: RUO
• Gene Alias: alpha-2a interferon; Ifa2; IFNA; IFN-a; IFNA 2; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFN-alpha 1b; IFN-alpha-1/13; IFN-alpha-2; IFN-alphaA; INFA2; Interferon; interferon alpha 1b; interferon alpha 2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; interferon alpha family, gene 2; interferon alpha-2; Interferon alpha-A; interferon alpha-D; interferon, alpha 2; Interferon-alpha II; leIF A; leIF D; RP11-354P17.2
• Common Name: Interferon alpha-2
• Gene Symbol: IFNA2
• Biological Activity: Specific Activity = 7.25 x 10e7 units/mg; The biological activity is determined by titration with the use of the cytopathic effect inhibition assay (Rubinstein et al., 1981). In this antiviral assay for interferon, about 1 unit/mL of interferon is the quantity necessary to inhibit the cytopathic effect by 50% using L929 cells challenged with encephalomyocarditis virus. The units are determined with respect to the international reference standard for mouse interferon alpha (mouse-IFN-α) provided by the National Institutes of Health (Pestka, 1986).
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Content And Storage: -80°C
• Activity: Specific Activity = 7.25x10e7 units/mg; The biological activity is determined by titration with the use of the cytopathic effect inhibition assay (Rubinstein et al., 1981). In this antiviral assay for interferon, about 1 unit/mL of interferon is the quantity necessary to inhibit the cytopathic effect by 50% using L929 cells challenged with encephalomyocarditis virus. The units are determined with respect to the international reference standard for mouse interferon alpha (mouse-IFN-α) provided by the National Institutes of Health (Pestka, 1986).
• Shipping Condition: Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Liquid
• Protein Subtype: Interferons
• Research Category: Immunology, Virology, Oncology, Signal Transduction
• Product Line: Gibco
• Purity or Quality Grade: ≥95%

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov