Invitrogen™ Mouse IgA Rapid ELISA Kit

Catalog No.	Quantity
EELR012	96 Tests

Catalog No. EELR012 Supplier Invitrogen™ Supplier No. EELR012

Description

ELISA

The Mouse Immunoglobulin A (IgA) ELISA quantitates IgA in serum and plasma. Principle of the method The Mouse IgA solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450±2 nm, is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

Specifications

• Accession Number: P01878
• Assay Range: 1.56-100 ng/mL
• Assay Sensitivity: 0.94 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IgA; Igh-2; Igha; immunoglobulin heavy constant alpha; ImmunoglobulinAIgA; Igh-2; Igha; immunoglobulin heavy constant alpha; ImmunoglobulinA;
• Gene ID (Entrez): 238447
• Gene Symbol: Igha
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Mouse IgA
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min