Invitrogen™ Mouse IgG Rapid ELISA Kit

Catalog No.	Quantity
EELR010	96 Tests

Catalog No. EELR010 Supplier Invitrogen™ Supplier No. EELR010

Description

ELISA

The Mouse Immunoglobulin G (IgG) ELISA quantitates IgG in serum and plasma. Principle of the method The Mouse IgG solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450±2 nm, is directly proportional to the concentration of target present in the original specimen. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Assay Range: 1.56-100 ng/mL
• Assay Sensitivity: 0.51 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; VH7183IgG1; Igh-4; Ighg1; Immunoglobulin G; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; VH7183;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Mouse IgG
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min