Invitrogen™ Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine5, eBioscience™

Catalog No.	Quantity
15-473-242	100 Tests

Catalog No. 15-473-242 Supplier Invitrogen™ Supplier No. 15473242

Description

Mouse Monoclonal Isotype Control

Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin. Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis. Applications Tested: Mouse IgG2b K Isotype Control PE-Cy5 has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG2b kappa
• Applications: Control, Flow Cytometry
• Classification: Monoclonal
• Clone: eBMG2b
• Concentration: 5 μL/Test
• Conjugate: PE-Cyanine5
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG2b κ

Safety and Handling

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