Gibco™ Mouse IL-10 ELISA Development Kit (TMB), PeproTech®

Catalog No.	Quantity
90-0T5-3K	10 x 96 Tests

Catalog No. 90-0T5-3K Supplier Gibco™ Supplier No. 900T53K

Description

ELISA

900-T53K consists of: 900-T53 (capture antibody, detection antibody, and protein standard), and HRP-17UL (streptavidin HRP-conjugate). Mouse IL-10 TMB ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinantmouse IL-10 in a sandwich ELISA format. Mouse IL-10 TMB ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells. The TMB ELISA Buffer Kit (Product # 900-T00), sold separately, is recommended to be used in conjunction with this TMB ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P18893
• Assay Range: Lot-specific
• Assay Sensitivity: Lot-specific
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF;
• Gene ID (Entrez): 16153
• Gene Symbol: IL10
• Kit Contents: This Mouse IL-10 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately., Detection Antibody, Protein Standard, HRP-conjugate
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Room temperature
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: IL-10
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min