Invitrogen eBioscience Mouse IL-15/IL-15R Complex ELISA Ready-SET-Go! Kit
For the quantitative detection of mouse IL-15/IL-15R complex in serum, plasma, and tissue culture supernatant samples.
Manufacturer: Invitrogen 88721522
DescriptionInterleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
|96-Well Plates: Corning Costar Flat-bottom (included with Cat. Nos. ending in -22 and -86)|
|2 x 96 tests|
|Serum,100 μL|Supernatant,100 μL|
|24 hr 30 min|
|Colorimetric Microplate Reader|
|1 hr 45 min|
|IL-15 Receptor complex with IL-15|
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