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Invitrogen™ eBioscience™ Mouse IL-15/IL-15R Complex ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

For the quantitative detection of mouse IL-15/IL-15R complex in serum, plasma, and tissue culture supernatant samples.

Manufacturer:  Invitrogen™ 88721522

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Catalog No. 50-112-8771


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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for the dilutions of antibodies and standards

Description

Description

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
Specifications

Specifications

P48346,Q60819
Biotin
16168, 16169
96-Well Plates: Corning Costar Flat-bottom (included with Cat. Nos. ending in -22 and -86)
2 x 96 tests
4 pg/mL
Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Il15, Il15ra
HRP
4-500 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Serum,Supernatant
Mouse
1 hr 45 min
Mouse
IL-15 Receptor complex with IL-15
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