Invitrogen™ Mouse IL-18 BPc ELISA Kit

Catalog No.
EM43RBX10	10 x 96 Tests; HRP

Catalog No. EM43RBX10 Supplier Invitrogen™ Supplier No. EM43RBX10

Description

ELISA

Mouse IL-18 BPc quantitates mouse IL-18 BPc in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse IL-18 BPc.

The IL18BPc gene encodes one of the isoforms of the interleukin-18 binding protein (IL-18BP), a crucial component in immune system regulation. Located in humans, IL18BPc is among four isoforms, alongside hIL-18BPa, b, and d, with hIL-18BPa being the predominant form. Both hIL-18BPa and hIL-18BPc effectively neutralize interleukin-18 (IL-18), a pro-inflammatory cytokine produced by T cells and NK cells, thereby mitigating its activity. This interaction is essential as IL-18 is implicated in several inflammatory and cardiovascular diseases. IL-18BP acts as a natural inhibitor, demonstrating a high affinity for IL-18, which makes it a significant focus for therapies targeting cytokine pathways in immunotherapy. Its ability to antagonize IL-18 highlights its potential as an immune checkpoint in cancer therapy, offering alternatives to enhance treatment efficacy and tolerability.

Specifications

• Assay Range: 22-5000 pg/mL
• Assay Sensitivity: 22 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: IL18 BPC; IL18BP; IL-18BP; IL-18BPC; Interleukin 18 binding proteinIL18 BPC; IL18BP; IL-18BP; IL-18BPC; Interleukin 18 binding protein;
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL18BPc
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min