Gibco™ Mouse IL-22 ELISA Development Kit (ABTS), PeproTech®

Catalog No.	Quantity
90-0K2-57K	10 x 96 Tests

Catalog No. 90-0K2-57K Supplier Gibco™ Supplier No. 900K257K

Description

ELISA

900-K257K consists of: 900-K257 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate). Mouse IL-22 ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant mouse IL-22 in a sandwich ELISA format. Mouse IL-22 ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells. The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.

Specifications

• Accession Number: Q9JJY9
• Assay Range: Lot-specific
• Assay Sensitivity: Lot-specific
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Cytokine Zcyto18; EGK_03903; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL22; IL-22; Il22a; IL-22a; IL-D110; ILN; ILTIF; IL-TIF; ILTIF alpha; IL-TIF alpha; ILTIFa; Interleukin; interleukin 10-related T cell-derived inducible factor; interleukin 22; Interleukin22; interleukin-22; Interleukin-22a; RGD1561292; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18Cytokine Zcyto18; EGK_03903; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL22; IL-22; Il22a; IL-22a; IL-D110; ILN; ILTIF; IL-TIF; ILTIF alpha; IL-TIF alpha; ILTIFa; Interleukin; interleukin 10-related T cell-derived inducible factor; interleukin 22; Interleukin22; interleukin-22; Interleukin-22a; RGD1561292; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18;
• Gene ID (Entrez): 50929
• Gene Symbol: IL22
• Kit Contents: This Mouse IL-22 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately., Detection Antibody, Protein Standard, HRP-conjugate
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Room temperature
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: IL-22
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min