Invitrogen™ Mouse IL-23 Recombinant Protein

Catalog No.	Quantity
50-112-8775	10 μg

Catalog No. 50-112-8775 Supplier Invitrogen™ Supplier No. 14823163

Description

Recombinant Protein

Description: IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-gamma. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. Applications Reported: Recombinant mouse IL-23 is biologically active. Applications Tested: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg. Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-Ser 355, accession # NM_008352 was co-expressed with mouse p19, amino acids met 1-Ala 196, accession # AF301619. Bioactivity...

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

Specifications

• Accession Number: Q9EQ14
• Concentration: 0.1 mg/mL
• For Use With (Application): Western Blot Control, Bioactivity, Functional Assay, Western Blot
• Formulation: PBS with BSA and no preservative; pH 6.0
• Gene ID (Entrez): 83430
• Molecular Weight (g/mol): 55.6 kDa
• Name: Mouse IL-23
• pH Range: 6.0
• Purification Method: Purified
• Quantity: 10 μg
• Source: Insect Cells
• Storage Requirements: -80°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.01 ng/μg
• Gene Alias: il 23; IL 23 A; IL12B; Il-12b; Il12p40; Il-12p40; il23; IL-23; IL-23 subunit alpha; IL23A; IL-23A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; P19; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798
• Common Name: IL-23 p19
• Gene Symbol: IL23A
• Biological Activity: ED50 < 625 pg/mL; as measured by the induction of IL-17 A in mouse splenocytes.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Sequence: Heterodimeric, mouse p40, amino acids Met1-Ser355 (Accession # NM_008352), and mouse p19, amino acids Met1-Ala196 (Accession # AF301619)
• Content And Storage: -80°C
• Activity: ED50 < 625 pg/mL; as measured by the induction of IL-17A in mouse splenocytes.
• Expression System: Insect cells
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >98% by SDS-PAGE

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov