Invitrogen™ eBioscience™ Mouse IL-27 ELISA Ready-SET-Go!™ Kit 

Engineered for accurate and precise measurement of mouse IL-27 protein levels from samples including serum, and cell culture supernatants.This ELISA has 11.8% cross reactivity to the recombinant human IL-27 and no cross reactivity to the recombinant mouse IL-12 when spiked in excess (100ng/ml)

Manufacturer:  Invitrogen™ 88727422

Catalog No. 50-173-05

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

Specifications

• Accession Number: Q8K3I6
• Conjugate: Biotin
• Gene ID (Entrez): 246779
• Kit Contents: 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
• Quantity: 2 x 96 tests
• Sensitivity: 16 pg/mL
• Sample Volume: Serum (100μL), Supernatants (100μL)
• Storage Requirements: 2-8°C
• Total Assay Time: 24 hr 30 min
• Gene Symbols: Il27
• Label or Dye: HRP

• Assay Range: 16-2,000 pg/mL
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA
• Regulatory Status: RUO
• Sample Type: Serum, Supernatants
• Species: Mouse
• Test Time: 1 hr 45 min
• Target: Mouse
• Synonym: Interleukin-27

For Research Use Only