Gibco™ Mouse IL-3, Animal-Free Recombinant Protein, PeproTech®

Catalog No.	Quantity
50-162-8478	250 μg
AF213132UG	2 μg
50-398-540	50 μg

Catalog No. 50-162-8478 Supplier Gibco™ Supplier No. AF21313250UG

Description

Recombinant Protein

Recombinant Murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Specifications

• Accession Number: P01586
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 16187
• Molecular Weight (g/mol): 15.1 kDa
• Name: Mouse IL-3, Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 250 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF
• Common Name: IL-3
• Gene Symbol: IL3
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse NFS-60 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC
• Content And Storage: -20°C
• Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse NFS-60 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.