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Gibco™ Mouse IL-3 ELISA Development Kit (ABTS), PeproTech®

Catalog No. 900K48K
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90-0K4-8K 10 x 96 Tests
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Catalog No. 90-0K4-8K Supplier Gibco™ Supplier No. 900K48K
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ELISA

900-K48K consists of: 900-K48 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate). Mouse IL-3 ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant mouse IL-3 in a sandwich ELISA format. Mouse IL-3 ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells. The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
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Specifications

Accession Number P01586
Assay Range Lot-specific
Assay Sensitivity Lot-specific
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Growth Factors & Receptors
Target Species Mouse
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSFBP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF;
Gene ID (Entrez) 16187
Gene Symbol IL3
Kit Contents This Mouse IL-3 ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately., Detection Antibody, Protein Standard, HRP-conjugate
Label or Dye Biotin
Quantity 10 x 96 Tests
Regulatory Status RUO
Shipping Condition Room temperature
Storage Requirements Refer to product documentation for component specific storage temperature.
Target IL-3
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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