Invitrogen™ Mouse IL-33 (ELISA Standard) Recombinant Protein

Catalog No.	Quantity
29-833-265	4 pack

Catalog No. 29-833-265 Supplier Invitrogen™ Supplier No. 29833265

Description

Recombinant Protein

Description: mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of 'alarmins' also known as endogeneous danger signals. Other known alarmins are IL-1 alpha; and HMGB1. IL-33 has been identified as the ligand for ST2 (a member of the Il-1 receptor family). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat. Applications Reported: This Mouse IL-33 ELISA Lyophilized Standard has been reported for use in the Mouse IL-33 Ready-Set-Go! ELISA kit (Product # 88-7333). Applications Tested: The Mouse IL-33 Lyophilized Standard is intended to be used as a standard for the Mouse IL-33 Ready-Set-Go! ELISA kit (Product # 88-7333-88).

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.

Specifications

• Accession Number: P14719, Q8BVZ5
• For Use With (Application): Neutralization, ELISA Standard
• Formulation: Protein with no preservative
• Gene ID (Entrez): 17082, 77125
• Molecular Weight (g/mol): 30 kDa
• Name: Mouse IL-33 (ELISA Standard)
• Purification Method: Purified
• Quantity: 4 pack
• Source: E. coli-expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33.
• Storage Requirements: 4°C
• Regulatory Status: RUO
• Gene Alias: 9230117N10Rik; C9orf26; DVS27; DVS27-related protein; H-IL-33; IL1F11; IL-1F11; Il33; IL-33; ILN; Interleukin; interleukin 33; interleukin-1 family member 11; Interleukin33; Interleukin-33; Interleukin-33 (109-270); Interleukin-33 (95-270); Interleukin-33 (99-270); Interleukin-33(102-266); Interleukin-33(109-266); NFEHEV; NFHEV; NF-HEV; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules; RGD1311155; RP11-575C20.2
• Common Name: IL-33
• Gene Symbol: IL1rl1, IL33
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Mouse IL-33, amino acids Ser109-Ile266
• Content And Storage: 4°C
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant