Invitrogen™ Mouse LBP ELISA Kit

Catalog No.	Quantity
EEL115	96 Tests

Catalog No. EEL115 Supplier Invitrogen™ Supplier No. EEL115

Description

ELISA

The Mouse LBP (Lipopolysaccharide Binding Protein) ELISA quantitates LBP in serum, plasma, and other biological fluids The Mouse LBP (Lipopolysaccharide Binding Protein) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The LBP protein is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Finally, this gene is found on chromosome 20, immediately downstream of the BPI gene.

Specifications

• Accession Number: Q61805
• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 1.88 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: BPI/LBP
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: acute phase protein; binds bacterial LPS; BPI fold containing family D, member 2; Bpifd2; hypothetical protein LOC512242; LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; lipopolysaccharide-binding protein LPSBP; LPS-binding protein; LPSBP; Ly88acute phase protein; binds bacterial LPS; BPI fold containing family D, member 2; Bpifd2; hypothetical protein LOC512242; LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; lipopolysaccharide-binding protein LPSBP; LPS-binding protein; LPSBP; Ly88;
• Gene ID (Entrez): 16803
• Gene Symbol: LBP
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: LBP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min