Invitrogen™ Mouse Luteinizing Hormone ELISA Kit

Catalog No.	Quantity
EEL121	96 Tests

Catalog No. EEL121 Supplier Invitrogen™ Supplier No. EEL121

Description

ELISA

The Mouse Luteinizing Hormone (LH) ELISA quantitates LH in serum, plasma, and other biological fluids The Mouse Luteinizing Hormone (LH) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Specifications

• Accession Number: O09108, P01216
• Assay Range: 0.31-20 ng/mL
• Assay Sensitivity: 0.19 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Glycoprotein hormone
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: aGSU; alphaGSU; alpha-GSU; alphaSU; Anterior pituitary glycoprotein hormones common subunit alpha; CGA; Cga1; CG-alpha; CGB4; Choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin; follitropin alpha chain; FSHA; FSH-alpha; FSH-B; FSH-beta; glycoprotein hormones alpha chain; glycoprotein hormones, alpha polypeptide; glycoprotein hormones, alpha subunit; GPHa; GPHA1; GPHalpha; HCG; HH23; hLHB; interstitial cell stimulating hormone, beta chain; interstitial cell-stimulating hormone; leutenising hormone beta; leutropin; LH; LH alpha 1-1; LH alpha 1-2; LH alpha 1-3; LH alpha 3; LH beta-1; LH beta-2; LH beta-3; LHA; LHB; LH-B; LHbeta; LSH-alpha; LSH-B; LSH-beta; Luteinizing hormone (lutropin) subunit beta; luteinizing hormone alpha chain; luteinizing hormone beta; luteinizing hormone beta polypeptide; luteinizing hormone beta subunit; luteinizing hormone subunit beta; Lutropin alpha chain; lutropin beta chain; Lutropin subunit beta; pituitary hormone alpha subunit; precursor polypeptide; precursor polypeptide (AA -24 to 96); thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSHA; TSH-alphaaGSU; alphaGSU; alpha-GSU; alphaSU; Anterior pituitary glycoprotein hormones common subunit alpha; CGA; Cga1; CG-alpha; CGB4; Choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin; follitropin alpha chain; FSHA; FSH-alpha; FSH-B; FSH-beta; glycoprotein hormones alpha chain; glycoprotein hormones, alpha polypeptide; glycoprotein hormones, alpha subunit; GPHa; GPHA1; GPHalpha; HCG; HH23; hLHB; interstitial cell stimulating hormone, beta chain; interstitial cell-stimulating hormone; leutenising hormone beta; leutropin; LH; LH alpha 1-1; LH alpha 1-2; LH alpha 1-3; LH alpha 3; LH beta-1; LH beta-2; LH beta-3; LHA; LHB; LH-B; LHbeta; LSH-alpha; LSH-B; LSH-beta; Luteinizing hormone (lutropin) subunit beta; luteinizing hormone alpha chain; luteinizing hormone beta; luteinizing hormone beta polypeptide; luteinizing hormone beta subunit; luteinizing hormone subunit beta; Lutropin alpha chain; lutropin beta chain; Lutropin subunit beta; pituitary hormone alpha subunit; precursor polypeptide; precursor polypeptide (AA -24 to 96); thyroid-stimulating hormone alpha chain; Thyrotropin alpha chain; TSHA; TSH-alpha;
• Gene ID (Entrez): 12640, 16866
• Gene Symbol: Cga, LHB
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Luteinizing Hormone
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min