Invitrogen™ Mouse MCP-1 ProQuantum Immunoassay Kit

Description

ELISA

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Specifications

• Accession Number: P10148
• Assay Range: 0.064 to 5000 pg/mL
• Assay Sensitivity: 0.06 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Immunoassay Kit Format: Proximity-based Immunoassay
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 20296
• Incubation Time: 1 hr.
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: Frozen at -20°C
• Target: CCL2 (MCP-1)
• Test Time: 30 mins
• Total Assay Time: 2 hrs