Invitrogen™ Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit with Plates

Catalog No.	Quantity
50-174-16	2 x 96 Tests
50-174-17	10 x 96 Tests

Catalog No. 50-174-16 Supplier Invitrogen™ Supplier No. 88750322

Description

ELISA

The Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCPT-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.

Specifications

• Accession Number: P11034
• Assay Range: 234-15000 pg/mL
• Assay Sensitivity: 234 pg/mL
• Content And Storage: Capture Antibody: Pre-titrated, purified antibody
  Detection Antibody: Pre-titrated, biotin-conjugated antibody
  Standard: Recombinant protein for generating standard curve and calibrating samples
  Coating Buffer: 10X PBS ELISA Coating Buffer
  Assay Diluent: 5X Concentrated
  Detection Enzyme: Pre-titrated Avidin-HRP
  Substrate Solution: Tetramethylbenzidine (TMB) Solution
  Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
  96-Well Plates: Corning Costar flat-bottom plated (included with catalog numbers ending in -22 and -86)
• Conjugate: HRP
• Immunoassay Kit Format: Uncoated ELISA Kit with Plates
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AV080368; mast cell protease 1; Mcp-1; Mcpt1; mMCP-1AV080368; mast cell protease 1; Mcp-1; Mcpt1; mMCP-1; CSIF; GVHDS; IL-10A; TGIF; IL10
• Gene ID (Entrez): 17224
• Gene Symbol: Mcpt1
• Kit Contents: Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Label or Dye: Biotin
• Protein Aliases: mMCP-1
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2-8°C
• Synonym: Mouse Mast Cell Protease-1
• Target: MCPT-1
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min

Safety and Handling

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