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Invitrogen™ eBioscience™ Mouse MCPT-1 (mMCP-1) ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Optimized for the accurate and precise measurement of mouse MCPT-1 in serum, and tissue culture supernatant samples. MCPT-1 (Mast Cell Protease-1) is a β-chymase, a type of serine protease stored and secreted in a tissue-specific manner by mucosal mast cells.

Manufacturer:  Invitrogen™ 88750322

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Catalog No. 50-174-16


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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for the dilution of antibodies and standards

Description

Description

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.
Specifications

Specifications

P11034
Biotin
17224
96-Well Plates: Corning Costar flat-bottom plated (included with catalog numbers ending in -22 and -86)
2 x 96 tests
120 pg/mL
Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
Mcpt1
HRP
120-15,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Mouse
1 hr 45 min
Mouse
Mouse Mast Cell Protease-1
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