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Sartorius Mouse Multi Ab Internalization
Supplier: Sartorius 97050
No wash protocol- fast easy antibody-labeling method optimal for screening antibody internalization.High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 L of cells and 10 L of labeled antibody kit .Simultaneously measure antibody internalization cell specificity and cell health within the same well.PrincipleAntibodies are easily labeled with Intellicyt Antibody Internalization Reagent a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.Cell viability can also be measured to assess general cell health and antibody function.
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