Invitrogen™ Mouse Neprilysin ELISA Kit

Catalog No.	Quantity
EM55RBX5	5 x 96 Tests
EM55RB	96 Tests
EM55RBX10	10 x 96 Tests

Catalog No. EM55RBX5 Supplier Invitrogen™ Supplier No. EM55RBX5

Description

ELISA

Mouse Neprilysin quantitates mouse Neprilysin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Neprilysin.

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA) and neutral endopeptidase (NEP), is a Zn2+-dependent metallo-peptidase with neutral metalloendopeptidase activity. It is a 100 kDa type II transmembrane glycoprotein encoded by a gene that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts, although the coding region remains unaffected by alternative splicing. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils and plays a role in stromal cell-dependent B lymphopoiesis. It is expressed on immature B cells in adult bone marrow, mature B cells in germinal centers, and cells from patients with chronic myelocytic leukemia (CML). Additionally, CD10 is present on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas. Beyond the hematopoietic compartment, CD10 is highly expressed on the brush border of enterocytes and renal tubules and glomeruli, as well as on breast myoepithelial cells, bile canaliculi, and fibroblasts. CD10 cleaves peptides at the amino side of hydrophobic residues, inactivating several biologically active peptides, including endothelin, glucagon, gastrin, neurotensin, bradykinin, enkephalins, substance P, oxytocin, and others. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie Tooth Disease, highlighting its significance in both normal physiological processes and disease states.

Specifications

• Accession Number: Q61391
• Assay Range: 61-15000 pg/mL
• Assay Sensitivity: 61 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; CMT2T; common acute lymphoblastic leukemia antigen; common acute lymphocytic leukemia antigen; Enkephalinase; EPN; membrane metallo endopeptidase; membrane metalloendopeptidase; membrane metallo-endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; MME; NEP; neprilysin; neprilysin-390; neprilysin-411; neutral endopeptidase 24.11; SFE; Skin fibroblast elastase6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; CMT2T; common acute lymphoblastic leukemia antigen; common acute lymphocytic leukemia antigen; Enkephalinase; EPN; membrane metallo endopeptidase; membrane metalloendopeptidase; membrane metallo-endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; MME; NEP; neprilysin; neprilysin-390; neprilysin-411; neutral endopeptidase 24.11; SFE; Skin fibroblast elastase;
• Gene ID (Entrez): 17380
• Gene Symbol: Mme
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: CD10
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov